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ERX1529094: Illumina HiSeq 2500 sequencing; qiita_ptid_1175:10308.J5.1.F
1 ILLUMINA (Illumina HiSeq 2500) run: 223,540 spots, 33.5M bases, 16Mb downloads

Design: fish gut samples from F. hetroclitus, F. diaphanus and F. majalis. Water and sediment from capture sites.
Submitted by: University of California San Diego Microbiome Initiative (University of California San Diego Microbiome Init)
Study: Patterns of microbiome transition along salinity gradients, and importance for fish host adaptive divergence across osmotic boundaries.
show Abstracthide Abstract
We are interested in whether the host fish microbiome is important for host adaptation to alternate osmotic niches. Toward this goal, we seek to describe the patterns of microbiome transition along an environmental salinity gradient.
Sample: Patterns of microbiome transition along salinity gradients, and importance for fish host adaptive divergence across osmotic boundaries.; 10308.J5.1.F
SAMEA4036386 • ERS1207496 • All experiments • All runs
Library:
Name: 10308.J5.1.F
Instrument: Illumina HiSeq 2500
Strategy: OTHER
Source: METAGENOMIC
Selection: PCR
Layout: SINGLE
Construction protocol: Illumina MiSeq 515fbc, 806r amplification of 16SrRNA V4
Experiment attributes: (show all 65 attributes...) (hide...)
altitude: 0
anonymized_name: J5-1-F
assigned_from_geo: False
barcode: GTCAACGCTGTC
body_habitat: not applicable
body_product: not applicable
body_site: not applicable
center_name: University of California San Diego Microbiome Init (show full text...)(hide...)
University of California San Diego Microbiome Initiative
center_project_name: Whitehead fish
collection_timestamp: 1/1/14 0:00
common_name: estuary metagenome
country: USA
depth: 0
description: Site J5 1 filtered water
dna_extracted: True
elevation: 0
emp_status: EMP
env_biome: estuarine biome
env_feature: large river biome
env_material: brackish water
env_matter: brackish water
env_package: water
experiment_center: UC Davis
experiment_title: Whitehead salinity series
extraction_robot: HOWE
extractionkit_lot: PS15F22
has_physical_specimen: True
host_common_name: estuary metagenome
host_subject_id: J5-1-F
host_taxid: 1649191
lane: lane 1
latitude: 36.99
linker: GT
longitude: -75.96
mastermix_lot: 151028862
orig_name: J5-1-F
pcr_primers: FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT
physical_specimen_location: UC Davis
physical_specimen_remaining: False
plating: LG
primer: GTGTGCCAGCMGCCGCGGTAA
primer_date: 73015
primer_plate: 1
processing_robot: ROBE
project_name: Whitehead_Fish
public: True
required_sample_info_status: completed
run_center: UCSDMI
run_date: 3/12/17
run_prefix: lane1_Undetermined_L001
samp_size: .1,g
sample_plate: WH_Fish_Plate1
sample_type: brackish water
scientific_name: estuary metagenome
sequencing_meth: Sequencing by synthesis
target_gene: 16S rRNA
target_subfragment: V4
taxon_id: 1649191
title: Patterns of microbiome transition along salinity g (show full text...)(hide...)
Patterns of microbiome transition along salinity gradients, and importance for fish host adaptive divergence across osmotic boundaries.
tm1000_8_tool: 108379Z
tm300_8_tool: 311313B
tm50_8_tool: 111075B
water_lot: RNBD2494
well_description: WH_Fish_Plate1_J5-1-F_E8
well_id: E8
Runs: 1 run, 223,540 spots, 33.5M bases, 16Mb
Run# of Spots# of BasesSizePublished
ERR1458616223,54033.5M16Mb2016-06-18

ID:
2649611

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